AMPK alpha 2 [p Ser491] Antibody (3H10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26401

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 2 Antibodies »

Product Summary for AMPK alpha 2 [p Ser491] Antibody (3H10)

Immunogen

A synthesized peptide derived from Human AMPK alpha 2 [p Ser491] [UniProt P54646]

Modification

p Ser 491

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK alpha 2 [p Ser491] Antibody (3H10)

AMPK alpha 2 [p Ser491] Antibody (3H10)

Western Blot: AMPK alpha 2 [p Ser491] Antibody (3H10) [NBP3-26401] -

Western Blot: AMPK alpha 2 [p Ser491] Antibody (3H10) [NBP3-26401] - Positive Western Blot detected in 293 whole cell lysate. A549 whole cell lysate.
All lanes: AMPK alpha 2 [p Ser491] Antibody at 1.1ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 62 KDa
Observed band size: 62 KDa

Applications for AMPK alpha 2 [p Ser491] Antibody (3H10)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: AMPK alpha 2

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Gene Symbol

PRKAA2

Additional AMPK alpha 2 Products

Product Documents for AMPK alpha 2 [p Ser491] Antibody (3H10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 2 [p Ser491] Antibody (3H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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