AMPK alpha 2 [p Ser345] Antibody (SR2089)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21563

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21563-100ul
NBP3-21563-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2089

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 2 Antibodies »

Product Summary for AMPK alpha 2 [p Ser345] Antibody (SR2089)

Immunogen

A synthesized peptide derived from human phospho-AMPK alpha 2 [Ser345] (Uniprot #: P54646)

Modification

p Ser 345

Specificity

Detects endogenous levels of phospho-AMPK alpha 2 [Ser345]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 2 [p Ser345] Antibody (SR2089)

Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] -

Western Blot: AMPK alpha 2 [p Ser 345] Antibody (SR2089) [NBP3-21563] - Western blot analysis using NBP3-21563 in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.

Applications for AMPK alpha 2 [p Ser345] Antibody (SR2089)

Application
Recommended Usage

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 2

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Gene Symbol

PRKAA2

Additional AMPK alpha 2 Products

Product Documents for AMPK alpha 2 [p Ser345] Antibody (SR2089)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 2 [p Ser345] Antibody (SR2089)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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