AMPK alpha 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49859

Novus Biologicals, part of Bio-Techne
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NBP1-49859
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all AMPK alpha 2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CPLDALNTTKP, from the internal region of the protein sequence according to NP_006243.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AMPK alpha 2 Antibody

Western Blot: AMPK alpha 2 Antibody [NBP1-49859]

Western Blot: AMPK alpha 2 Antibody [NBP1-49859]

Western Blot: AMPK alpha 2 Antibody [NBP1-49859] - Staining of Mouse (A) and Rat (B) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: AMPK alpha 2 Antibody [NBP1-49859]

Western Blot: AMPK alpha 2 Antibody [NBP1-49859]

Western Blot: AMPK alpha 2 Antibody [NBP1-49859] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for AMPK alpha 2 Antibody

Application
Recommended Usage

Peptide ELISA

1:16000

Western Blot

0.3 - 1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK alpha 2

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Entrez Gene IDs

5563 (Human); 108079 (Mouse); 78975 (Rat)

Gene Symbol

PRKAA2

UniProt

NP_006243.2 (Human)

Additional AMPK alpha 2 Products

Product Documents for AMPK alpha 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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