AMPK alpha 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38532
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: IDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AMPK alpha 2 Antibody
Western Blot: AMPK alpha 2 Antibody [NBP2-38532]
Western Blot: AMPK alpha 2 Antibody [NBP2-38532] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MGImmunocytochemistry/ Immunofluorescence: AMPK alpha 2 Antibody [NBP2-38532]
Immunocytochemistry/Immunofluorescence: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human cell line HEK 293 shows localization to nuclear speckles & the Golgi apparatus.Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532]
Immunohistochemistry-Paraffin: AMPK alpha 2 Antibody [NBP2-38532] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.Applications for AMPK alpha 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AMPK alpha 2
Long Name
AMP-activated Protein Kinase alpha
Alternate Names
PRKAA2
Gene Symbol
PRKAA2
UniProt
Additional AMPK alpha 2 Products
Product Documents for AMPK alpha 2 Antibody
Product Specific Notices for AMPK alpha 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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