AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16943

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK beta 1 Antibodies »

Product Summary for AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around S182 of human AMPK beta 1 (NP_006244.2). SSSPP

Modification

p Ser 182

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

Western Blot: AMPK beta 1 [p Ser182] AntibodyAzide and BSA Free [NBP3-16943]

Western Blot: AMPK beta 1 [p Ser182] AntibodyAzide and BSA Free [NBP3-16943]

Western Blot: AMPK beta 1 [p Ser182] Antibody [NBP3-16943] - Western blot analysis of extracts of C2C12 cells, using AMPK beta 1 antibody (NBP3-16943) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 uM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free [NBP3-16943]

Immunohistochemistry-Paraffin: AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free [NBP3-16943]

Immunohistochemistry-Paraffin: AMPK beta 1 [p Ser182] Antibody [NBP3-16943] - Immunohistochemistry of paraffin-embedded mouse testis using AMPK beta 1 Rabbit pAb (NBP3-16943) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK beta 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).

Long Name

AMP-activated Protein Kinase beta 1

Alternate Names

PRKAB1

Gene Symbol

PRKAB1

Additional AMPK beta 1 Products

Product Documents for AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK beta 1 [p Ser182] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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