Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H8

Format

BSA Free

Concentration

1.0 mg/ml

View all Androgen R/NR3C4 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Androgen Receptor expressed in E. coli. [UniProt# P10275]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Androgen R/NR3C4 Antibody (2H8) - BSA Free

Western Blot: Androgen R/NR3C4 Antibody (2H8)BSA Free [NBP1-47471]

Western Blot: Androgen R/NR3C4 Antibody (2H8)BSA Free [NBP1-47471]

Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysates.
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) - BSA Free [NBP1-47471]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) - BSA Free [NBP1-47471]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis of paraffin-embedded human Prostate tissues using Androgen Receptor mouse mAb
Western Blot: Androgen R/NR3C4 Antibody (2H8)BSA Free [NBP1-47471]

Western Blot: Androgen R/NR3C4 Antibody (2H8)BSA Free [NBP1-47471]

Western Blot: Androgen R/NR3C4 Antibody (2H8) [NBP1-47471] - Analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.
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Applications for Androgen R/NR3C4 Antibody (2H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This Androgen Receptor (2H8) antibody is useful in Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Androgen R/NR3C4

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the Androgen Receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The Androgen Receptor is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Androgen R/NR3C4 Antibody (2H8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Androgen R/NR3C4 Antibody (2H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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