Androgen R/NR3C4 Antibody (PO00-29)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32035
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PO00-29
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Androgen R/NR3C4 aa 300-400 (N terminal). (Uniprot: P10275)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Androgen R/NR3C4 Antibody (PO00-29)
Western Blot: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035]
Western Blot: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035] - Western blot analysis of Androgen R/NR3C4 on different lysates with Rabbit anti-Androgen R/NR3C4 antibody (NBP3-32035) at 1/1,000 dilution.Lane 1: A549 cell lysate
Lane 2: K-562 cell lysate
Lane 3: 22RV1 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 99 kDa
Observed band size: 99/75 kDa
Exposure time: 10 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32035) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035]
Immunohistochemistry: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Androgen R/NR3C4 antibody (NBP3-32035) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32035) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035]
Immunohistochemistry: Androgen R/NR3C4 Antibody (PO00-29) [NBP3-32035] - Immunofluorescence analysis of paraffin-embedded rat epididymis tissue labeling Androgen R/NR3C4 with Rabbit anti-Androgen R/NR3C4 antibody (NBP3-32035) at 1/50 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32035, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for Androgen R/NR3C4 Antibody (PO00-29)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:200-1:1000-1:200
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Androgen R/NR3C4
Long Name
Androgen Receptor
Alternate Names
AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1
Gene Symbol
AR
Additional Androgen R/NR3C4 Products
Product Documents for Androgen R/NR3C4 Antibody (PO00-29)
Product Specific Notices for Androgen R/NR3C4 Antibody (PO00-29)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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