Androgen R/NR3C4 Antibody (ST0453)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67497

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67497
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST0453

Concentration

1 mg/ml

View all Androgen R/NR3C4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Androgen R/NR3C4 aa 595-644 / 644. (SwissProt: P10275 Human)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Androgen R/NR3C4 Antibody (ST0453)

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497] - Staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow Cytometry: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Flow Cytometry: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Flow Cytometry: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497] - Analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497]

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody (ST0453) [NBP2-67497] - Staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications for Androgen R/NR3C4 Antibody (ST0453)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Androgen R/NR3C4

Androgens have a broad range of effects on the appearance and maintenance of male secondary sexual characteristics. Like other members of the steroid superfamily, the androgen receptor (AR) consists of an amino terminal modulating domain, a central DNA binding domain, a hinge region and a carboxy terminal ligand binding domain. The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Gene Symbol

AR

Additional Androgen R/NR3C4 Products

Product Documents for Androgen R/NR3C4 Antibody (ST0453)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Androgen R/NR3C4 Antibody (ST0453)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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