Androgen R/NR3C4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1446

Novus Biologicals, part of Bio-Techne
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NB100-1446
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Key Product Details

Species Reactivity

Validated:

Human, Equine, Feline

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Androgen R/NR3C4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence EVQLGLGRVYPRPPSC corresponding to N-Terminus according to NP_000035.2.

Reactivity Notes

Feline and Equine reactivity reported from a verified customer review.

Localization

Nuclear

Specificity

This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Androgen R/NR3C4 Antibody

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446]

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446]

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446] - Staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446]

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446]

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446] - (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Androgen R/NR3C4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2-3 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1-0.5ug/ml
Application Notes
ChIP: A 6-fold increased presence of the FKBP5 locus observed when comparing the precipitations using Goat Anti-Androgen Receptor with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
WB: Approx. 95 kDa band observed in human brain and human heart lysates (calculated MW of 99 kDa band according to NP_000035).
IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.8 using NB100-1446 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Androgen R/NR3C4

Like all steroid receptors, the androgen receptor has several functional domains. Androgen receptors have a DNA binding domain, a hinge section, and the hormone binding domain. There is only one active form of the androgen receptor. Smaller forms represent proteolytic fragments that are not considered physiologically relevant. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human); 11835 (Mouse); 24208 (Rat)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Androgen R/NR3C4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Androgen R/NR3C4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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