Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70172

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Angiopoietin-1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 198-498 of human ANGPT1 (NP_001137) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free

Western Blot: Angiopoietin-1 Antibody (OTI8H2)Azide and BSA Free [NBP2-70172]

Western Blot: Angiopoietin-1 Antibody (OTI8H2)Azide and BSA Free [NBP2-70172]

Western Blot: Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free [NBP2-70172] - Human recombinant protein fragment corresponding to amino acids 198-498 of human ANGPT1 (NP_001137) produced in E.coli.
Immunohistochemistry: Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free [NBP2-70172]

Immunohistochemistry: Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free [NBP2-70172]

Immunohistochemistry: Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free [NBP2-70172] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Angiopoietin-1 Antibody (OTI8H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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