Anillin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1314

Novus Biologicals, part of Bio-Techne
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NB100-1314
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Anillin Antibodies »

Product Specifications

Immunogen

Peptide with sequence WQPDACYKPIGKP corresponding to C-Terminus according to NP_061155.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Anillin Antibody

Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NB100-1314]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NB100-1314]

Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314]

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314]

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314] - 10ug/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry: Anillin Antibody [NB100-1314]

Flow Cytometry: Anillin Antibody [NB100-1314]

Flow Cytometry: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Anillin Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 10 ug/ml

Immunohistochemistry-Paraffin

3 - 10 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in mouse brain lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124 kDa band according to NP_061155.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of nuclei in some cells of renal tubules.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. Anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Entrez Gene IDs

54443 (Human); 68743 (Mouse)

Gene Symbol

ANLN

UniProt

NP_061155.2 (Human)

Additional Anillin Products

Product Documents for Anillin Antibody

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Product Specific Notices for Anillin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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