Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Anillin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1074 and 1124 of human actin binding protein Anillin using the numbering given in entry NP_061155.2 (GeneID 54443).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anillin Antibody

Western Blot: Anillin Antibody [NB100-68216]

Western Blot: Anillin Antibody [NB100-68216]

Western Blot: Anillin Antibody [NB100-68216] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68216 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Anillin was also immunoprecipitated by NB100-68215, which recognizes an upstream epitope.
Immunohistochemistry: Anillin Antibody [NB100-68216]

Immunohistochemistry: Anillin Antibody [NB100-68216]

Immunohistochemistry: Anillin Antibody [NB100-68216] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Anillin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .
Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216]

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216]

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-Anillin used at a dilution of 1:250.

Applications for Anillin Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Entrez Gene IDs

54443 (Human)

Gene Symbol

ANLN

UniProt

NP_061155.2 (Human)

Additional Anillin Products

Product Documents for Anillin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Anillin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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