ANKRD28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41419

Novus Biologicals, part of Bio-Techne
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NB100-41419
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ANKRD28 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human ankyrin repeat domain 28 (phosphatase interactor targeting K protein) using the numbering given in entry NP_056014.2 (GeneID 23243).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANKRD28 Antibody

Western Blot: ANKRD28 Antibody [NB100-41419]

Western Blot: ANKRD28 Antibody [NB100-41419]

Western Blot: ANKRD28 Antibody [NB100-41419] - Whole cell lysate from HeLa cells (5, 15 and 50 mcg for WB; 1 mg for IP, 20 percent of IP loaded) and 293T (T; 50 mcg) cells. NB100-41419 used for WB at 0.02 mug/ml (A) and 1 mug/ml (B) and used for IP at 3 mcg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody NB100-41420, which recognizes a downstream epitope.
Immunohistochemistry: ANKRD28 Antibody [NB100-41419]

Immunohistochemistry: ANKRD28 Antibody [NB100-41419]

Immunohistochemistry: ANKRD28 Antibody [NB100-41419] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419]

Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419]

Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419] - FFPE section of human stomach carcinoma. Antibody used at a dilution of 1:250.

Applications for ANKRD28 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD28

PITK was previously identified as a novel PP1 targeting subunit implicated in modulating the phosphorylation of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K). Through the phosphorylation of PITK at S1013 and S1017 (residues that flank or reside within a PP1C-binding motif), the binding of the PP1 catalytic subunit to PITK, and subsequently the activity of the holoenzyme, are discretely controlled.

Alternate Names

ankyrin repeat domain 28, Ankyrin repeat domain-containing protein 28, KIAA0379PITKPP6-ARS-Aphosphatase interactor targeting K protein, Phosphatase interactor targeting protein hnRNP K, protein phosphatase 6 ankyrin repeat subunit A, serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A

Entrez Gene IDs

23243 (Human)

Gene Symbol

ANKRD28

UniProt

O15084 (Human)

Additional ANKRD28 Products

Product Documents for ANKRD28 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ANKRD28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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