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ANKRD65 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39062

Novus Biologicals, part of Bio-Techne
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NBP2-39062
NBP2-39062-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EEEQELRWMELDSEEALGTRTEGPSVVQGWGHLLQAVWRGPAGLVTQLLRQGH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD65 Antibody

Western Blot: ANKRD65 Antibody [NBP2-39062]

Western Blot: ANKRD65 Antibody [NBP2-39062]

Western Blot: ANKRD65 Antibody [NBP2-39062] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: ANKRD65 Antibody [NBP2-39062]

Immunocytochemistry/ Immunofluorescence: ANKRD65 Antibody [NBP2-39062]

Immunocytochemistry/Immunofluorescence: ANKRD65 Antibody [NBP2-39062] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ANKRD65 Antibody [NBP2-39062]

Immunohistochemistry-Paraffin: ANKRD65 Antibody [NBP2-39062]

Immunohistochemistry-Paraffin: ANKRD65 Antibody [NBP2-39062] - Staining in human fallopian tube and kidney tissues using anti-ANKRD65 antibody. Corresponding ANKRD65 RNA-seq data are presented for the same tissues.

Applications for ANKRD65 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD65

Alternate Names

ankyrin repeat domain 65, hypothetical protein LOC441869

Gene Symbol

ANKRD65

UniProt

Additional ANKRD65 Products

Product Documents for ANKRD65 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANKRD65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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