Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S388A-10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ankyrin 1 Antibodies »

Product Summary for Ankyrin 1 Antibody (S388A-10)

Immunogen

Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R

Localization

Cytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line

Specificity

Detects 200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Ankyrin 1 Antibody (S388A-10)

Western Blot: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Western Blot: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Western Blot: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10 (NBP2-59315). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody (NBP2-59315) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
Immunocytochemistry/ Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Immunocytochemistry/ Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Immunocytochemistry/Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 1 Monoclonal Antibody, Clone S388A-10 (NBP2-59315). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 1 Monoclonal Antibody (NBP2-59315) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Immunocytochemistry/ Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315]

Immunocytochemistry/Immunofluorescence: Ankyrin 1 Antibody (S388A-10) [NBP2-59315] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.

Applications for Ankyrin 1 Antibody (S388A-10)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Ankyrin 1

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actincytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and themaintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various targetproteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed ofthree structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highlyconserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subjectto variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has alsobeen found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of allpatients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving riseto different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting fromusage of an alternate promoter have also been identified. (provided by RefSeq)

Alternate Names

ANK, ANK-1, ankyrin 1, erythrocytic, ankyrin-1, ankyrin-R, Erythrocyte ankyrin, SPH1, SPH2

Gene Symbol

ANK1

Additional Ankyrin 1 Products

Product Documents for Ankyrin 1 Antibody (S388A-10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ankyrin 1 Antibody (S388A-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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