Ankyrin 3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05550
![Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]](https://resources.bio-techne.com/images/products/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG1
Concentration
1 mg/ml
Product Specifications
Immunogen
The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG1
Scientific Data Images for Ankyrin 3 Antibody
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]
Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.![Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]](https://resources.bio-techne.com/images/products/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]")
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550]
Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Immunofluorescent analysis of a rat brain section stained with Ankyrin 3 Antibody at a dilution of 1:1,000, in red and costained with mouse mAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 antibody stains axonal initial segments, while the MAP2 antibody labels MAP2 protein expressed in the perikarya and dendrites of most neurons.Applications for Ankyrin 3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: Ankyrin 3
Alternate Names
ankyrin G, Ankyrin G119, ankyrin-3, ankyrin-G, Node Of Ranvier
Gene Symbol
ANK3
Additional Ankyrin 3 Products
Product Documents for Ankyrin 3 Antibody
Product Specific Notices for Ankyrin 3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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