Annexin A1 Antibody (ANXA1/3566)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07716

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ANXA1/3566

Concentration

0.2 mg/ml

View all Annexin A1 Antibodies »

Product Summary for Annexin A1 Antibody (ANXA1/3566)

Immunogen

Recombinant full-length human Annexin A1 protein (Uniprot: P04083)

Localization

Nuclear, Cytoplasm and Cell Surface

Marker

Hairy Cell Leukemia Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08708)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Annexin A1 Antibody (ANXA1/3566)

Western Blot: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Western Blot: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Western Blot: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716] - Western Blot Analysis of A549 cell lysate using Annexin A1 Mouse Monoclonal Antibody (Clone ANXA1/3566).
Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716] - Immunofluorescence staining of paraformaldehyde-fixed HeLa cells with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti- Mouse IgG-CF488 (Green). Nuclei are labeled with RedDot (Red).
Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716]

Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-07716] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).
View 5 more images

Applications for Annexin A1 Antibody (ANXA1/3566)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Annexin A1

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Alternate Names

ANX1, ANXA1, LPC1

Gene Symbol

ANXA1

UniProt

P04083

Additional Annexin A1 Products

Product Documents for Annexin A1 Antibody (ANXA1/3566)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Annexin A1 Antibody (ANXA1/3566)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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