Annexin V Antibody (3B9B7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52414

Novus Biologicals, part of Bio-Techne
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NBP2-52414
NBP2-52414-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B9B7

Format

BSA Free

Concentration

1 mg/ml

View all Annexin V Antibodies »

Product Summary for Annexin V Antibody (3B9B7) - BSA Free

Immunogen

Purified recombinant fragment of human Annexin V (AA: 160-320) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Annexin V Antibody (3B9B7) - BSA Free

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Immunocytochemistry/Immunofluorescence: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - An intracellular stain was performed on HeLa cells with Annexin V Antibody (3B9B7) NBP2-52414AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Applications for Annexin V Antibody (3B9B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin V

Annexin V is encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

Additional Annexin V Products

Product Documents for Annexin V Antibody (3B9B7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Annexin V Antibody (3B9B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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