AOF1 Antibody (JE32-40)

Western Blot: AOF1 Antibody (JE32-40) [NBP3-32547]

Western Blot: AOF1 Antibody (JE32-40) [NBP3-32547] - Western blot analysis of AOF1 on different lysates with Rabbit anti-AOF1 antibody (NBP3-32547) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A549 cell lysate
Lane 4: K-562 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: U-2 OS cell lysate
Lane 7: HCT 116 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32547) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.