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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A6/2/2

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Peptide to C-Terminal of AP2 alpha coupled to KLH. PNSHTDNNAKSSDKEEKHRK

Localization

Nuclear

Specificity

This antibody detects both the alpha and beta forms of AP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AP2 alpha Antibody (A6/2/2) - BSA Free

Flow Cytometry: AP2 alpha Antibody (A6/2/2) - BSA Free [NB600-202]

Flow Cytometry: AP2 alpha Antibody (A6/2/2) - BSA Free [NB600-202]

Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for AP2 alpha Antibody (A6/2/2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Radioimmunoassay

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
Recommended for immunohistology using formal saline fixative with calcium on paraffin embedded specimens (10min microwave treatment required). This antibody is CyTOF ready. Positive control(s): Raji cells or breast carcinomas.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2 alpha

AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM]

Alternate Names

Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha

Entrez Gene IDs

7020 (Human)

Gene Symbol

TFAP2A

Additional AP2 alpha Products

Product Documents for AP2 alpha Antibody (A6/2/2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AP2 alpha Antibody (A6/2/2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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