Key Product Details

Species Reactivity

Validated:

Human

Applications

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AP2a 8G8/5

Format

BSA Free

Concentration

0.82 mg/ml

View all AP2 alpha Antibodies »

Product Summary for AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

Immunogen

Bacterialy expressed AP-2a/AP-2g fusion protein.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Antigen recovery using microwave or pressure cooking required for paraffin embedded tissue. Positive control(s): Extract from breast cancer cell line ZR75-1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2 alpha

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

Alternate Names

Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha

Entrez Gene IDs

7020 (Human)

Gene Symbol

TFAP2A

Additional AP2 alpha Products

Product Documents for AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.