AP2 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38809

Novus Biologicals, part of Bio-Techne
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NBP2-38809
NBP2-38809-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AP2 alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GQRQSQESGLLHTHRGLPHQLSGLDP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP2 alpha Antibody

Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809]

Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809]

Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809] - Staining in human placenta and colon tissues using anti-TFAP2A antibody. Corresponding TFAP2A RNA-seq data are presented for the same tissues.
Western Blot: AP2 alpha Antibody [NBP2-38809]

Western Blot: AP2 alpha Antibody [NBP2-38809]

Western Blot: AP2 alpha Antibody [NBP2-38809] - Analysis using Anti-TFAP2A antibody NBP2-38809 (A) shows similar pattern to independent antibody NBP1-90143 (B).
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody [NBP2-38809]

Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody [NBP2-38809]

Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody [NBP2-38809] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
View 2 more images

Applications for AP2 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2 alpha

AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM]

Alternate Names

Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha

Gene Symbol

TFAP2A

UniProt

P05549 (Human)

Additional AP2 alpha Products

Product Documents for AP2 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AP2 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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