AP2A2 Antibody (6D5F5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33317
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6D5F5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human AP2A2 (NP_036437.1).
Sequence:
STDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF
Sequence:
STDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AP2A2 Antibody (6D5F5)
Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] -
Western Blot: AP2A2 Antibody (6D5F5) [NBP3-33317] - Western blot analysis of various lysates using AP2A2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Applications for AP2A2 Antibody (6D5F5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:2000 - 1:20000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AP2A2
Alternate Names
100 kDa coated vesicle protein C, Adapter-related protein complex 2 alpha-2 subunit, adaptin, alpha B, Adaptor protein complex AP-2 subunit alpha-2, adaptor-related protein complex 2, alpha 2 subunit, alpha2-adaptin, Alpha-adaptin C, alpha-adaptin C; Huntingtin interacting protein J, AP-2 complex subunit alpha-2, Clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, DKFZP564D1864, HIP9ADTAB, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, HYPJCLAPA2, KIAA0899HIP-9, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Gene Symbol
AP2A2
Additional AP2A2 Products
Product Specific Notices for AP2A2 Antibody (6D5F5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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