AP2A2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21648
![Western Blot: AP2A2 Antibody [NBP2-21648]](https://resources.bio-techne.com/images/products/AP2A2%20Antibody-Western%20Blot-NBP2-21648-img0002.jpg "Western Blot: AP2A2 Antibody [NBP2-21648]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for AP2A2 Antibody
Immunogen
This affinity purified AP2A2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins. (Uniprot: O95782)
Reactivity Notes
A BLAST analysis was used to suggest reactivity with AP2A21 and AP2A22 protein from human, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Cross reactivity with AP2A2 proteins from other sources is not known.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This affinity purified antibody is directed against human AP2A2. The product was affinity purified from antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for AP2A2 Antibody
![Western Blot: AP2A2 Antibody [NBP2-21648]](https://resources.bio-techne.com/images/products/AP2A2 Antibody-Western Blot-NBP2-21648-img0002.jpg "Western Blot: AP2A2 Antibody [NBP2-21648]")
Western Blot: AP2A2 Antibody [NBP2-21648]
Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: HeLa, Lane 3: 293, Lane 4: A431 and Lane 5: Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approx. 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.Applications for AP2A2 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: AP2A2
Alternate Names
100 kDa coated vesicle protein C, Adapter-related protein complex 2 alpha-2 subunit, adaptin, alpha B, Adaptor protein complex AP-2 subunit alpha-2, adaptor-related protein complex 2, alpha 2 subunit, alpha2-adaptin, Alpha-adaptin C, alpha-adaptin C; Huntingtin interacting protein J, AP-2 complex subunit alpha-2, Clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, DKFZP564D1864, HIP9ADTAB, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, HYPJCLAPA2, KIAA0899HIP-9, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Gene Symbol
AP2A2
UniProt
Additional AP2A2 Products
Product Documents for AP2A2 Antibody
Product Specific Notices for AP2A2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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