Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AP2M1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1(NP_001020376) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2M1 Antibody (OTI1E9) - Azide and BSA Free

Western Blot: AP2M1 Antibody (OTI1E9)Azide and BSA Free [NBP2-70417]

Western Blot: AP2M1 Antibody (OTI1E9)Azide and BSA Free [NBP2-70417]

Western Blot: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417]

Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417]

Immunocytochemistry/Immunofluorescence: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.
Immunohistochemistry: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417]

Immunohistochemistry: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417]

Immunohistochemistry: AP2M1 Antibody (OTI1E9) - Azide and BSA Free [NBP2-70417] - Staining of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody.
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Applications for AP2M1 Antibody (OTI1E9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2M1

AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. It is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.

Alternate Names

Adaptin-mu2, adaptor-related protein complex 2, mu 1 subunit, AP-2 mu chain, Clathrin assembly protein complex 2 medium chain, clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, HA2 50 kDa subunit, KIAA0109, mu subunit, Plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein

Gene Symbol

AP2M1

Additional AP2M1 Products

Product Documents for AP2M1 Antibody (OTI1E9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AP2M1 Antibody (OTI1E9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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