Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 13F11

Format

BSA Free

Concentration

1 mg/ml

View all APAF-1 Antibodies »

Product Specifications

Immunogen

Recombinant mouse APAF-1 (aa 1-97) containing the N-terminal CARD domain.

Specificity

Recognizes the CARD domain of mouse and rat APAF-1. Does not cross-react with human APAF-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for APAF-1 Antibody (13F11) - BSA Free

Western Blot: APAF-1 Antibody (13F11)BSA Free [NBP2-80093]

Western Blot: APAF-1 Antibody (13F11)BSA Free [NBP2-80093]

Western Blot: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093] - Analysis using Apaf-1 (mouse/rat), MAb (13F11) (NBP2-80093) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
Immunoprecipitation: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093]

Immunoprecipitation: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093]

Immunoprecipitation: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093] - On mouse thymocytes using APAF-1 (mouse/rat), MAb (13F11) (NBP2-80093).

Applications for APAF-1 Antibody (13F11) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APAF-1

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol

APAF1

Additional APAF-1 Products

Product Documents for APAF-1 Antibody (13F11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APAF-1 Antibody (13F11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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