APAF-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56074
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Gerbil
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Full-length recombinant human Apaf1 protein was used as immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for APAF-1 Antibody
Western Blot: APAF-1 Antibody [NB100-56074]
Western Blot: APAF1 Antibody [NB100-56074] - Analysis of Apaf 1 using this antibody. Jurkat: human T-cell leukemia cell line lysate. RS11-846: human B-cell leukemia cell line lysate. Human thymus: tissue lysate.Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody [NB100-56074]
Immunocytochemistry/Immunofluorescence: APAF1 Antibody [NB100-56074] - Formalin-fixed, paraffin-embedded sections from a human brain tumor tissue microarray stained for Apaf 1 expression using NB100-56074 at 1:2000. A and B. Tissue microarray overview. A1 and B1, higher magnification of cores from A and B, respectively. Differential expression of Apaf 1 is seen between patient samples. Hematoxylin-eosin counterstain.Immunohistochemistry: APAF-1 Antibody [NB100-56074]
Immunohistochemistry: APAF-1 Antibody [NB100-56074] - APAF1 Antibody [NB100-56074] - Formalin-fixed, paraffin-embedded tissue sections of human brain tumors stained for Apaf 1 expression using NB100-56074 at 1:2000. A. Gemistocytoma (grade II). B. Anaplastic glioma. A high level of Apaf 1 was observed in the gemistocytoma whereas a low level was seen in the more malignant anaplastic glioma. Hematoxylin-eosin counterstain.Applications for APAF-1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:50-1:200
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Neat whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: APAF-1
Long Name
Apoptotic Peptidase Activating Factor 1
Alternate Names
APAF1
Gene Symbol
APAF1
UniProt
Additional APAF-1 Products
Product Documents for APAF-1 Antibody
Product Specific Notices for APAF-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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