Apc1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37901

Novus Biologicals, part of Bio-Techne
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NBP2-37901
NBP2-37901-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Apc1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc1 Antibody

Western Blot: Apc1 Antibody [NBP2-37901]

Western Blot: Apc1 Antibody [NBP2-37901]

Western Blot: Apc1 Antibody [NBP2-37901] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: Apc1 Antibody [NBP2-37901]

Immunocytochemistry/ Immunofluorescence: Apc1 Antibody [NBP2-37901]

Immunocytochemistry/Immunofluorescence: Apc1 Antibody [NBP2-37901] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Apc1 Antibody [NBP2-37901]

Immunohistochemistry-Paraffin: Apc1 Antibody [NBP2-37901]

Immunohistochemistry-Paraffin: Apc1 Antibody [NBP2-37901] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for Apc1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc1

APC1 is 1 of at least 11 subunits of the anaphase promoting complex (APC), which functions at the metaphase to anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Alternate Names

anaphase promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), anaphase-promoting complex subunit 1, APC1Testis-specific gene 24 protein, Cyclosome subunit 1, Mitotic checkpoint regulator, TSG24MCPR

Gene Symbol

ANAPC1

UniProt

Q9H1A4 (Human)

Additional Apc1 Products

Product Documents for Apc1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apc1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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