Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Apc11 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ANAPC11(NP_001002244) produced in HEk293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apc11 Antibody (OTI3F5) - Azide and BSA Free

Western Blot: Apc11 Antibody (OTI3F5)Azide and BSA Free [NBP2-71939]

Western Blot: Apc11 Antibody (OTI3F5)Azide and BSA Free [NBP2-71939]

Western Blot: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Apc11.
Immunocytochemistry/ Immunofluorescence: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939]

Immunocytochemistry/ Immunofluorescence: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939]

Immunocytochemistry/Immunofluorescence: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC11.
Immunohistochemistry: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939]

Immunohistochemistry: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939]

Immunohistochemistry: Apc11 Antibody (OTI3F5) - Azide and BSA Free [NBP2-71939] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for Apc11 Antibody (OTI3F5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apc11

APC11 (anaphase-promoting complex subunit 11) is a member of the E3 enzyme family. This protein contains a RING-H2 domain and has a molecular weight of approximately 9.8 kD. The APC11 protein is distributed diffusely in the cytoplasm and is located in the nucleus with discrete accumulation in granular structures. The APC11 protein is a probable catalytic unit in the APC complex. The APC11 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC11 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. In addition to the APC complex proteins, APC11 has been shown to interact with Ubc4. The Poly6116 antibody has been shown to be useful for Western blotting of the human and mouse APC11 protein.

Alternate Names

anaphase promoting complex subunit 11, anaphase promoting complex subunit 11 (yeast APC11 homolog), anaphase-promoting complex subunit 11, APC11 anaphase promoting complex subunit 11 homolog, APC11Hepatocellular carcinoma-associated RING finger protein, Apc11p, Cyclosome subunit 11, HSPC214, MGC882

Gene Symbol

ANAPC11

Additional Apc11 Products

Product Documents for Apc11 Antibody (OTI3F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apc11 Antibody (OTI3F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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