Apc4 Antibody

Western Blot: Apc4 Antibody [NB100-59824]

Western Blot: Apc4 Antibody [NB100-59824] - Detection of Human and Mouse APC4 on HeLa whole cell lysates using NB100-59824. APC4 was also immunoprecipitated by rabbit anti-APC4 antibody NB100-59823.

Knockout Validated: Rabbit Polyclonal Apc4 Antibody [NB100-59824]

Knockout Validated: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Imaging overexpressed HA-APC4. Scale bars: 10 μm. (A) HEK293 cells stained with DAPI (blue), GFP (green), HA (magenta), APC4 (red). (B) Wildtype primary mouse hippocampal cultures stained with DAPI (blue), MAP2 (magenta), HA (red), APC4 (green). 1:500 with 36-40hr primary incubation (24 or less does not work for both HEK293 and neurons). The signal that is there is absent in knockout cells. Image from a verified customer review.

Knockout Validated: Rabbit Polyclonal Apc4 Antibody [NB100-59824]

Knockout Validated: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Primary hippocampal neuron cultures were generated from tm1c/tm1c conditional ANAPC4 knockout mice and infected at DIV1 with a lentivirus expressing Cre RFP P2A or a No Infection control. Coverslips were fixed at DIV11 and stained with antibodies against MAP2 (blue), Cre (green), and APC4 (magenta). The RFP expression was also detected (red). The settings on the confocal microscope were determined by using a non-infected control sample that was not stained with the APC4 primary antibody (Secondary Only). Scale bars representing 10 um are displayed on the merged images (Merge). Image from a verified customer review.