Apc7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40367

Novus Biologicals, part of Bio-Techne
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NBP1-40367
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Apc7 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 515 and 565 of human anaphase promoting complex subunit 7 using the numbering given in entry AAI41849.1 (GeneID 51434).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc7 Antibody

Western Blot: Apc7 Antibody [NBP1-40367]

Western Blot: Apc7 Antibody [NBP1-40367]

Western Blot: Apc7 Antibody [NBP1-40367] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-APC7 antibody NBP1-40367 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. APC7 was also immunoprecipitated by rabbit anti-APC7 antibody NBP1-40366, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Immunohistochemistry: Apc7 Antibody [NBP1-40367]

Immunohistochemistry: Apc7 Antibody [NBP1-40367]

Immunohistochemistry: Apc7 Antibody [NBP1-40367] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-APC7 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for Apc7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apc7

APC7 (anaphase-promoting complex subunit 7) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 63 kD. The APC7 protein is located in the nucleus during interphase and the centrosome during metaphase/anaphase. This protein probably recruits Cdh1 into the APC complex. The APC7 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC7 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The Poly6113 antibody has been shown to be useful for Western blotting of the human APC7 protein.

Alternate Names

anaphase promoting complex subunit 7, anaphase-promoting complex subunit 7, APC7Cyclosome subunit 7

Entrez Gene IDs

51434 (Human)

Gene Symbol

ANAPC7

Additional Apc7 Products

Product Documents for Apc7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Apc7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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