APE Antibody (3W5N10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15361
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3W5N10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for APE Antibody (3W5N10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human APE (P27695). MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for APE Antibody (3W5N10)
![Western Blot: APE Antibody (3W5N10) [NBP3-15361]](https://resources.bio-techne.com/images/products/APE-Antibody-3W5N10-Western-Blot-NBP3-15361-img0002.jpg "Western Blot: APE Antibody (3W5N10) [NBP3-15361]")
Western Blot: APE Antibody (3W5N10) [NBP3-15361]
Western Blot: APE Antibody (3W5N10) [NBP3-15361] - Western blot analysis of extracts of various cell lines, using APEX1/APE Rabbit mAb (NBP3-15361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: APE Antibody (3W5N10) [NBP3-15361]](https://resources.bio-techne.com/images/products/APE-Antibody-3W5N10-Immunohistochemistry-Paraffin-NBP3-15361-img0001.jpg "Immunohistochemistry-Paraffin: APE Antibody (3W5N10) [NBP3-15361]")
Immunohistochemistry-Paraffin: APE Antibody (3W5N10) [NBP3-15361]
Immunohistochemistry-Paraffin: APE Antibody (3W5N10) [NBP3-15361] - Immunohistochemistry of paraffin-embedded human colon using APEX1/APE Rabbit mAb (NBP3-15361) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for APE Antibody (3W5N10)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: APE
For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.
APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.
APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.
Long Name
Apurinic/Apyrimidinic Endonuclease
Alternate Names
APEX1
Gene Symbol
APEX1
Additional APE Products
Product Documents for APE Antibody (3W5N10)
Product Specific Notices for APE Antibody (3W5N10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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