APE Antibody (CPTC-APEX1-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07864

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CPTC-APEX1-2

Concentration

0.2 mg/ml

View all APE Antibodies »

Product Summary for APE Antibody (CPTC-APEX1-2)

Immunogen

Recombinant human full-length APE protein (Uniprot: P27695)

Localization

Mitochondrion, Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08370)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for APE Antibody (CPTC-APEX1-2)

Immunocytochemistry/ Immunofluorescence: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Immunocytochemistry/ Immunofluorescence: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Immunocytochemistry/Immunofluorescence: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Immunofluorescence Analysis of human HeLa cells labeling APE with APE Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by goat anti- Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.
Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APE Mouse Monoclonal Antibody (CPTC-APEX1-2).
Flow Cytometry: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Flow Cytometry: APE Antibody (CPTC-APEX1-2) [NBP3-07864]

Flow Cytometry: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Flow Cytometric Analysis of HeLa cells using APE Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for APE Antibody (CPTC-APEX1-2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: APE

DNA-(apurinic or apyrimidinic site) lyase, also known as APE1 or APEX1, is a multifunctional mammalian proteins responsible for the cellular response to oxidative stress. Specifically, APE-1 both functions in the DNA base excision repair pathway and regulates the redox of transcriptional factors.

For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.

APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.

APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol

APEX1

UniProt

P27695

Additional APE Products

Product Documents for APE Antibody (CPTC-APEX1-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APE Antibody (CPTC-APEX1-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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