APE Antibody (S07-5A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15113
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S07-5A9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for APE Antibody (S07-5A9)
Immunogen
A synthetic peptide of human APE (Uniprot#: P27695)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for APE Antibody (S07-5A9)
![Western Blot: APE Antibody (S07-5A9) [NBP3-15113]](https://resources.bio-techne.com/images/products/APE-Antibody-S07-5A9-Western-Blot-NBP3-15113-img0001.jpg "Western Blot: APE Antibody (S07-5A9) [NBP3-15113]")
Western Blot: APE Antibody (S07-5A9) [NBP3-15113]
Western Blot: APE Antibody (S07-5A9) [NBP3-15113] - Western blot detection of APE in K562, C6, 3T3, Hela cell lysates using APE Rabbit mAb (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.Applications for APE Antibody (S07-5A9)
Application
Recommended Usage
Western Blot
1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: APE
For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.
APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.
APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.
Long Name
Apurinic/Apyrimidinic Endonuclease
Alternate Names
APEX1
Gene Symbol
APEX1
Additional APE Products
Product Documents for APE Antibody (S07-5A9)
Product Specific Notices for APE Antibody (S07-5A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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