APE Antibody - BSA Free

Western Blot: APE AntibodyBSA Free [NB100-101]

Western Blot: APE Antibody [NB100-101] - RKO cells were subjected to scrambled siRNA (Mock) or siRNA against TDP1 (TDP1KD) and cell lysates analyzed by immunoblotting (left). Control RKO (RKOSc siRNA) and RKO cells in which TDP1 levels were depleted (RKOTDP1 siRNA) were examined for their survival following exposure to the indicated doses of MMS, as described earlier in the text (right). Increasing the load of unrepaired methylated purines by exploiting the limited availability of TDP1 alone or in combination with canonical BER factors such as APE1 provides a new synthetic lethal setting to improve the clinical outcome of temozolomide-based cancer therapy. Image collected and cropped by CiteAb from the following publication (academic.oup.com/nar/article/42/5/3089/1051107), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: APE Antibody - BSA Free [NB100-101]

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - IF on HeLa cells. Image from verified customer review.

Western Blot: APE AntibodyBSA Free [NB100-101]

Western Blot: APE Antibody [NB100-101] - SH-SY5Y (nuclear extract) tested at 1:1000 dilution. Image provided by verified customer review.

Flow Cytometry: APE Antibody - BSA Free [NB100-101]

Flow Cytometry: APE Antibody [NB100-101] - Spectral properties of Alexa Fluor(R) 488: an excitation maximum of 490 nm and an emission maximum of 525 nm. Use the Novus Spectra Viewer to identify the optimal laser(s) and filters for Alexa Fluor(R) 488 and to determine its compatibility with other fluorophores when designing a multicolor experiment. https://www.novusbio.com/spectraviewer

Western Blot: APE AntibodyBSA Free [NB100-101]

Western Blot: APE Antibody [NB100-101] - Analysis of APE in human melanoma cell lysate using anti-APE antibody. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: APE Antibody - BSA Free [NB100-101]

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - Detection of APE1 (Green) in HepG2 cells using NB100-101. Nuclei (Blue) are counterstained with Hoechst 33258.

Western Blot: APE Antibody - BSA Free [NB100-101] -

Western Blot: APE Antibody - BSA Free [NB100-101] - Defective p53 activity leads to failure of the BER coordination system. (A) High-throughput microscopy analysis of TIG-1 cells transfected with plasmids expressing wild-type or mutant p53. Boxplots showing the distribution of APE1 staining intensity (in arbitrary units) in p53 low versus p53 overexpressing cells. Each p53 mutant is reported on top of the relevant plot. The dashed line highlights the median APE1 intensity in p53 low cells (N > 5000). NS: not statistically significant at P < 0.05. (B) Representative western blotting analysis comparing WI38 & WI38 (SV40) cells upon transfection with the indicated siRNAs. Failure to downregulate Sp1 correlates with the inability to modulate APE1. Actin was used as loading control. (C) qPCR analysis on APE1 transcript in WI38 & WI38 (SV40) cells upon transfection with the indicated siRNAs. APE1 transcription is reduced in WI38 cells only. Note the higher transcript content in WI38 (SV40) cells. (D) Representative Western blotting analysis comparing WI38 & WI38 (SV40) cells upon transfection with the indicated siRNAs. Failure to modulate BER correlates with gammaH2AX staining in WI38 (SV40) cells; gammaH2AX increases further upon XRCC1 depletion. (E) Neutral Comet assay on WI38 & WI38 (SV40) fibroblasts shows accumulation of DSBs upon XRCC1-depletion in transformed cells only. Results are expressed as mean ± SD of three independent experiments. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody - BSA Free [NB100-101] -

Western Blot: APE Antibody - BSA Free [NB100-101] - APE1 expression in HCC tissues(A) qPCR of tumor (HCC), peri-HCC, & surrounding liver cirrhosis (SLC), & normal liver (CTRL) (left panel). Ratio between HCC & SLC & HCC & peri-HCC within the same patients (right panel). APE1 mRNA quantification was normalized to two reference genes 18srRNA & Actin. Bar graphs indicate mean & SEM. (B) APE1 protein quantification in HCC & SLC tissue lysates from HCC cancer patients. Graphs indicate the different distributions of the fold of protein expression for each sample as the ratio between APE1 & actin. (see Supplementary Figure 1). *P < 0.05. (C) Western blot analysis of HCC & SLC tissue lysates patients performed on pooled samples from HCC cancer. Actin was used as loading control & for the relative normalization. A representative image of western blot analysis is shown. Data represent the means of ± SD of three independent experiments. *P < 0.05. (D) Immunohistochemistry of HCC, SLC, & normal (CTRL) tissue. Red & yellow arrows indicate nuclear & cytoplasmic positivity, respectively. (E) Scan of HCC nodules & its corresponding sAPE1 from 3 patients representing for each low, median, & high sAPE1. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30719231), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody - BSA Free [NB100-101] -

Western Blot: APE Antibody - BSA Free [NB100-101] - Persistent SSBs decrease APE1 levels by affecting its transcription. (A) Western blotting analysis on a representative XRCC1 knockdown time-course. TIG-1 cells were incubated with either a control siRNA (72 h), or a XRCC1-targeting siRNA, & harvested at the indicated time points. DNA damage accumulation is highlighted by PAR formation; APE1 is downregulated in a time-dependent manner. Actin was used as loading control. (B) Alkaline Comet assay on TIG-1 cells harvested 72 h after XRCC1 depletion shows accumulation of SSBs (N = 9). (C) Histogram showing the downregulation in APE1 protein amount 72 h after XRCC1 knockdown, as measured by western blotting (N = 10). (D) Histogram showing the downregulation in APE1 transcript level 72 h after XRCC1 depletion, as measured by qPCR (N = 7). Results depicted in histograms are presented as mean ± SD of the indicated number (N) of independent experiments. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody - BSA Free [NB100-101] -

Western Blot: APE Antibody - BSA Free [NB100-101] - Downregulation of APE1 in response to BER unbalance is dependent of p53. (A) Representative western blotting analysis on TIG-1 cells depleted of XRCC1 & p53. APE1 is downregulated upon XRCC1 knockdown in a p53-dependent manner. Actin was used as loading control. (B) Left: histogram showing the quantification of APE1 protein amount in the experiment showed in panel A (N = 5). Right: histogram illustrating the amount of APE1 transcript upon combined XRCC1/p53 depletion, as measured through qPCR (N = 3). Results are expressed as mean ± SD of the indicated number (N) of independent experiments. (C) Boxplot showing the distribution of APE1 staining intensity (in arbitrary units) in p53 low versus p53 overexpressing cells. The dashed line highlights the median APE1 intensity in p53 low cells (N > 8000). (D) Representative high-throughput immuno-fluorescence pictures showing TIG-1 cells stained for APE1 (right panel) & p53 (middle panel) after transfection with a p53 expressing plasmid. Cells that downregulating APE1 in response to p53 overexpression are marked by a contour line. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: APE Antibody - BSA Free [NB100-101] -

Western Blot: APE Antibody - BSA Free [NB100-101] - Excessive AP-endonuclease activity in cells with p53 impairment leads to accumulation of genomic instability. (A) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gammaH2AX staining upon co-depletion of XRCC1 & APE1. (B) Neutral Comet assay on WI38 (SV40) fibroblasts shows accumulation of DSBs upon XRCC1-depletion & rescue after co-depletion of XRCC1 & APE1. Results are expressed as mean ± SD of three independent experiments. (C) Representative western blotting analysis on WI38 (SV40) cells shows rescue of gammaH2AX staining after depletion of XRCC1 in presence of APE1 inhibitor III (APE1i III, 5 μM for 24 h), or AR03 (2.5 μM for 24 h). Actin was used as loading control in all western blotting experiments. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.