APE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56359

Novus Biologicals, part of Bio-Techne
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NB100-56359
NB100-56359SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all APE Antibodies »

Product Summary for APE Antibody

Immunogen

This antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 36-52 and 216-232 of human APE.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APE Antibody

Western Blot: APE Antibody [NB100-56359]

Western Blot: APE Antibody [NB100-56359]

Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: APE Antibody [NB100-56359]

Immunohistochemistry-Paraffin: APE Antibody [NB100-56359]

Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.

Applications for APE Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/mL

Western Blot

2 - 5 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

Human apurinic (apyrimidinic) endonuclease/redox-factor 1 (APE/Ref-1) is involved in the repair of DNA damage as well as in the transcriptional regulation of genes. The repair function of the protein is located on its C-terminal region. Activation of transcription factors, which occurs via a redox-based mechanism, pertains to a approximately 6 kDa N-terminal fragment with Cys-65 being of essential importance. Ape1/ref-1 has been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression such as Fos, Jun, NF (B, PAX, HIF-1(, HLF and p53. APE/Ref-1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Thus, specific downmodulation of APE/Ref-1 activity in tumors might be considered as a novel therapeutic approach in tumor therapy.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Entrez Gene IDs

328 (Human)

Gene Symbol

APEX1

UniProt

NP_001632 (Human)

Additional APE Products

Product Documents for APE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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