APE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-897

Novus Biologicals, part of Bio-Techne
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NB100-897
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all APE Antibodies »

Product Specifications

Immunogen

Peptide with sequence PKRGKKGAVAEDGD-C corresponding to N-Terminus according to NP_001632.2.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33753167).

Specificity

Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for APE Antibody

Western Blot: APE Antibody [NB100-897]

Western Blot: APE Antibody [NB100-897]

Western Blot: APE Antibody [NB100-897] - Staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: APE Antibody [NB100-897]

Immunohistochemistry-Paraffin: APE Antibody [NB100-897]

Immunohistochemistry-Paraffin: APE Antibody [NB100-897] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Applications for APE Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4 - 6 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in nuclear lysates of A431, HeLa and MCF7 (calculated MW of 35.6 kDa band according to NP_001632.2, NP_542379.1 and NP_542380.1).Use in ICC/IF reported in scientific literature (PMID: 27560983).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APE

Apurinic / apyrimidinic endonuclease (APE1 or REF1) is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. Alterations in APE1 levels have been described in some cancers. APE1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Entrez Gene IDs

328 (Human)

Gene Symbol

APEX1

UniProt

NP_001632 (Human)

Additional APE Products

Product Documents for APE Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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