APEH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85333

Novus Biologicals, part of Bio-Techne
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NBP1-85333
NBP1-85333-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all APEH Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%). Mouse reactivity reported in scientific literature (PMID: 24554718).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APEH Antibody

Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333]

Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333]

Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining in human kidney and pancreas tissues using anti-APEH antibody. Corresponding APEH RNA-seq data are presented for the same tissues.
Western Blot: APEH Antibody [NBP1-85333]

Western Blot: APEH Antibody [NBP1-85333]

Western Blot: APEH Antibody [NBP1-85333] - Analysis using Anti-APEH antibody NBP1-85333 (A) shows similar pattern to independent antibody NBP1-85332 (B).
Immunocytochemistry/ Immunofluorescence: APEH Antibody [NBP1-85333]

Immunocytochemistry/ Immunofluorescence: APEH Antibody [NBP1-85333]

Immunocytochemistry/Immunofluorescence: APEH Antibody [NBP1-85333] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
View 5 more images

Applications for APEH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24554718). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APEH

The APEH gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated aminoacid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is furtherprocessed to acetate and a free

Alternate Names

AARE, ACPH, acylamino-acid-releasing enzyme, acylaminoacyl-peptidase, Acyl-peptide hydrolase, APH, D3F15S2, D3S48EEC 3.4.19.1, DNF15S2, MGC2178, N-acylaminoacyl-peptide hydrolase, OPH, Oxidized protein hydrolase

Gene Symbol

APEH

Additional APEH Products

Product Documents for APEH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APEH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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