APEX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56625

Novus Biologicals, part of Bio-Techne
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NB100-56625
NB100-56625SS
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all APEX2 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with EELRALDSEGRALLTQHKI (aa122-140) and GQAKTSEAKDEKELRTSF (aa438-455) peptides of human APEX2.

Reactivity Notes

Predicted cross-reactivity based on sequence identity (not validated): Mouse, Rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APEX2 Antibody

Western Blot: APEX2 Antibody [NB100-56625]

Western Blot: APEX2 Antibody [NB100-56625]

Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.

Applications for APEX2 Antibody

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
In HeLa, a 54 kDa band is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APEX2

Apurinic/apyrimidinic (AP) sites occur frequently in DNA by spontaneous hydrolysis, DNA damaging agents or DNA glycosylases that remove specific abnormal bases. ClassII AP endonucleases act to repair this damage by cleaving the phosphodiester backbone. It is primarily located in the nucleus but is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).

Alternate Names

AP endonuclease 2, APE2, APEX nuclease (apurinic/apyrimidinic endonuclease) 2, APEX nuclease 2, APEXL2, Apurinic-apyrimidinic endonuclease 2, DNA-(apurinic or apyrimidinic site) lyase 2, DNA-apurinic or apyrimidinic site lyase 2, EC 3.1, EC 4.2.99.18, XTH2

Entrez Gene IDs

27301 (Human)

Gene Symbol

APEX2

UniProt

Q9UBZ4 (Human)

Additional APEX2 Products

Product Documents for APEX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APEX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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