API5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89326

Novus Biologicals, part of Bio-Techne
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NBP1-89326
NBP1-89326-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all API5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for API5 Antibody

Western Blot: API5 Antibody [NBP1-89326]

Western Blot: API5 Antibody [NBP1-89326]

Western Blot: API5 Antibody [NBP1-89326] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: API5 Antibody [NBP1-89326]

Immunocytochemistry/ Immunofluorescence: API5 Antibody [NBP1-89326]

Immunocytochemistry/Immunofluorescence: API5 Antibody [NBP1-89326] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: API5 Antibody [NBP1-89326]

Immunohistochemistry-Paraffin: API5 Antibody [NBP1-89326]

Immunohistochemistry-Paraffin: API5 Antibody [NBP1-89326] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
View 1 more images

Applications for API5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: API5

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Alternate Names

AAC-11API5L1, AAC11fibroblast growth factor 2-interacting factor 2, Antiapoptosis clone 11 protein, API-5, apoptosis inhibitor 5, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, migration-inducing protein MIG8, Protein XAGL

Gene Symbol

API5

Additional API5 Products

Product Documents for API5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for API5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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