APOBEC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93372
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EAASQNGEDLENLDD corresponding to internal region (near N-Terminus) according to NP_006780.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for APOBEC2 Antibody
![Western Blot: APOBEC2 Antibody [NB100-93372]](https://resources.bio-techne.com/images/products/APOBEC2-Antibody-Western-Blot-NB100-93372-img0002.jpg "Western Blot: APOBEC2 Antibody [NB100-93372]")
Western Blot: APOBEC2 Antibody [NB100-93372]
Western Blot: APOBEC2 Antibody [NB100-93372] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for APOBEC2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:1000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in human heart and skeletal muscle lysates (calculated MW of 25.7 kDa band according to NP_006780.1).
An additional band of 90 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: APOBEC2
Alternate Names
apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, EC 3.5.4, EC 3.5.4.-, probable C->U-editing enzyme APOBEC-2
Gene Symbol
APOBEC2
UniProt
Additional APOBEC2 Products
Product Documents for APOBEC2 Antibody
Product Specific Notices for APOBEC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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