APOBEC3B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99174
![Western Blot: APOBEC3B Antibody [NBP2-99174]](https://resources.bio-techne.com/images/products/APOBEC3B-Antibody-Western-Blot-NBP2-99174-img0002.jpg "Western Blot: APOBEC3B Antibody [NBP2-99174]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for APOBEC3B Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human APOBEC3B.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for APOBEC3B Antibody
Western Blot: APOBEC3B Antibody [NBP2-99174]
Western Blot: APOBEC3B Antibody [NBP2-99174] - Anti-APOBEC3B rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: APOBEC3B Antibody [NBP2-99174]](https://resources.bio-techne.com/images/products/APOBEC3B-Antibody-Immunoprecipitation-NBP2-99174-img0001.jpg "Immunoprecipitation: APOBEC3B Antibody [NBP2-99174]")
Immunoprecipitation: APOBEC3B Antibody [NBP2-99174]
Immunoprecipitation: APOBEC3B Antibody [NBP2-99174] - APOBEC3B was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-APOBEC3B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-APOBEC3B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDaApplications for APOBEC3B Antibody
Application
Recommended Usage
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: APOBEC3B
Alternate Names
APOBEC1L, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B, ARCD3, ARP4, bK150C2.2, cytidine deaminase, DJ742C19.2, EC 3.5.4, EC 3.5.4.-, FLJ21201, phorbolin 3, Phorbolin-1-related protein, Phorbolin-2/3, PHRBNLphorbolin 2, probable DNA dC->dU-editing enzyme APOBEC-3B
Gene Symbol
APOBEC3B
Additional APOBEC3B Products
Product Documents for APOBEC3B Antibody
Product Specific Notices for APOBEC3B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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