Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E12

Format

BSA Free

Concentration

1 mg/ml

View all Apolipoprotein A-I/ApoA1 Antibodies »

Product Summary for Apolipoprotein A-I/ApoA1 Antibody (1E12) - BSA Free

Immunogen

Recombinant human Apolipoprotein A-I/ApoA1 (25-267aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody (1E12) - BSA Free

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (1E12)BSA Free [NBP1-97943]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (1E12)BSA Free [NBP1-97943]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (1E12) [NBP1-97943] - The extracts of HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for Apolipoprotein A-I/ApoA1 Antibody (1E12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (APOA1) is the main protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in APOA1 are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. High level of APOA1 is regarded as protective against atherosclerosis and cardiovascular disease.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

UniProt

NP_000030.1 (Human)

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody (1E12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody (1E12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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