Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2083D

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Apolipoprotein A-I/ApoA1 Antibodies »

Product Summary for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Immunogen

rhApoA1 aa25-267

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D)Azide and BSA Free [MAB3664]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D)Azide and BSA Free [MAB3664]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - Analysis of HepG2 cell lysate with 0.1 ug/ml of hApoA1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunocytochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed HepG2 cell using ApoA1 Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free [MAB3664]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody (2083D) [MAB3664] - ApoA1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using ApoA1 Monoclonal Antibody at 1 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.
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Applications for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody (2083D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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