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Apolipoprotein B/ApoB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-7616

Novus Biologicals, part of Bio-Techne
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NB120-7616

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Apolipoprotein B/ApoB was isolated from human plasma by density gradient centrifugation followed by HPLC purification. (Uniprot: Q7Z7Q0)

Reactivity Notes

Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible

Localization

Secreted

Specificity

Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Apolipoprotein B/ApoB Antibody

Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616]

Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616]

Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616] - Apolipoprotein B/ApoB antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.

Applications for Apolipoprotein B/ApoB Antibody

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:50-1:500

Immunoprecipitation

1:100

Sandwich ELISA

1:100-1:2000

Western Blot

1:200-1:1000
Application Notes
This product have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Use in Sandwich-ELISA reported in scientific literature (PMID 26648662)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Apolipoprotein B/ApoB

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein antibody is derived from human plasma by density gradient ultracentrifugation and HPLC.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human)

Gene Symbol

APOB

UniProt

Additional Apolipoprotein B/ApoB Products

Product Documents for Apolipoprotein B/ApoB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein B/ApoB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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