Apolipoprotein B/ApoB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7616
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Apolipoprotein B/ApoB was isolated from human plasma by density gradient centrifugation followed by HPLC purification. (Uniprot: Q7Z7Q0)
Reactivity Notes
Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible
Localization
Secreted
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Apolipoprotein B/ApoB Antibody
Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616]
Western Blot: Apolipoprotein B/ApoB Antibody [NB120-7616] - Coommassie stained gel showing both free and HDL bound Apolipoprotein B/ApoB eluted from a solid phase resin prepared using anti-Human Apolipoprotein B/ApoB antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616]
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NB120-7616] - Apolipoprotein B/ApoB antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.Applications for Apolipoprotein B/ApoB Antibody
Application
Recommended Usage
ELISA
1:2000-1:10000
Immunohistochemistry
1:50-1:500
Immunoprecipitation
1:100
Sandwich ELISA
1:100-1:2000
Western Blot
1:200-1:1000
Application Notes
This product have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Use in Sandwich-ELISA reported in scientific literature (PMID 26648662)
Use in Sandwich-ELISA reported in scientific literature (PMID 26648662)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: Apolipoprotein B/ApoB
Alternate Names
APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4
Entrez Gene IDs
338 (Human)
Gene Symbol
APOB
UniProt
Additional Apolipoprotein B/ApoB Products
Product Documents for Apolipoprotein B/ApoB Antibody
Product Specific Notices for Apolipoprotein B/ApoB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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