Apolipoprotein C-II/ApoC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB400-155
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Apolipoprotein C-II/ApoC2 Antibody
Immunogen
Apolipoprotein C-II/ApoC2 produced synthetically in full-length form (not selected epitopes) using conventional peptide technology. (Uniprot: P02655)
Reactivity Notes
Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-II and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Apolipoprotein C-II/ApoC2 Antibody
Immunohistochemistry: Apolipoprotein C-II/ApoC2 Antibody [NB400-155]
Immunohistochemistry: Apolipoprotein C-II/ApoC2 Antibody [NB400-155] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: Apolipoprotein C-II at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-II as precipitated red signal with hematoxylin purple nuclear counterstain.Apolipoprotein C-II/ApoC2 Antibody
Immunohistochemistry of Apolipoprotein C-II/ApoC2 Antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-II at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-II as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for Apolipoprotein C-II/ApoC2 Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1:100
Western Blot
1:5000-1:10000
Application Notes
This product have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: Apolipoprotein C-II/ApoC2
Additional Apolipoprotein C-II/ApoC2 Products
Product Documents for Apolipoprotein C-II/ApoC2 Antibody
Product Specific Notices for Apolipoprotein C-II/ApoC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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