Apolipoprotein D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42526

Novus Biologicals, part of Bio-Techne
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NBP2-42526
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Apolipoprotein D Antibodies »

Product Specifications

Immunogen

Peptide with sequence EIEKIPTTFENGRC corresponding to internal region according to NP_001638.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein D Antibody

Western Blot: Apolipoprotein D Antibody [NBP2-42526]

Western Blot: Apolipoprotein D Antibody [NBP2-42526]

Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Figure 4. ApoA1, ApoD (Apolipoprotein D), and LCAT immunolabeling in cultured keratocytes, dermal fibroblasts, and HepG2 liver cells. Cells were immunolabeled with ApoD (d-f) antibodies (green). DAPI was used to visualize DNA (blue). Anti-ApoD immunolabeled keratocytes (d) and dermal fibroblasts (e), but not HepG2 cells (f). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785/htm) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Apolipoprotein D Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Use in Flow reported in scientific literature (PMID:33334888). WB: Approx. 25 kDa band observed in human liver and kidney lysates (calculated MW of 21.3 kDa band according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Use in ICC/IF was reported in scientific literature (PMID: 31779197).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein D

The Apolipoprotein D gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoproteinsequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2microglobulin protein superfamily

Alternate Names

ApoD, Apo-D, apolipoprotein D

Entrez Gene IDs

347 (Human)

Gene Symbol

APOD

Additional Apolipoprotein D Products

Product Documents for Apolipoprotein D Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Apolipoprotein D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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