Apolipoprotein E/ApoE Antibody (E981)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18297
Key Product Details
Species Reactivity
Non-human Primate
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # E981
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Purified human apolipoprotein E/ApoE
Reactivity Notes
The monoclonal antibody reacts with human apoE and cross-react with apoE from non-human primates.
Specificity
Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Apolipoprotein E/ApoE Antibody (E981)
Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297]
Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.Applications for Apolipoprotein E/ApoE Antibody (E981)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: Apolipoprotein E/ApoE
Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.
The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.
Alternate Names
APOE
Gene Symbol
APOE
Additional Apolipoprotein E/ApoE Products
Product Documents for Apolipoprotein E/ApoE Antibody (E981)
Product Specific Notices for Apolipoprotein E/ApoE Antibody (E981)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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