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Apolipoprotein E/ApoE Antibody (E981)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18297

Novus Biologicals, part of Bio-Techne
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NBP3-18297-1000ug
NBP3-18297-250ug

Key Product Details

Species Reactivity

Non-human Primate

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # E981

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Purified human apolipoprotein E/ApoE

Reactivity Notes

The monoclonal antibody reacts with human apoE and cross-react with apoE from non-human primates.

Specificity

Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Apolipoprotein E/ApoE Antibody (E981)

Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297]

Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297]

Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

Applications for Apolipoprotein E/ApoE Antibody (E981)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered PBS

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (E981)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein E/ApoE Antibody (E981)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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