Apolipoprotein E/ApoE Antibody (GT27711)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13692
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT27711
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E/ApoE. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Apolipoprotein E/ApoE Antibody (GT27711)
![ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692]](https://resources.bio-techne.com/images/products/Apolipoprotein-E-ApoE-Antibody-GT27711-ELISA-NBP3-13692-img0001.jpg "ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692]")
ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692]
ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) (1 ug/mL). Mouse IgG antibody (HRP) (NBP2-19382) (1:10000) was used to detect bound primary antibody.
Sandwich ELISA: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) as capture antibody at concentration of 5 ug/mL and Apolipoprotein E/ApoE antibody [C2C3], C-term as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] -
Western Blot: Apolipoprotein E/ApoE Antibody (GT27711) [NBP3-13692] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E/ApoE antibody [GT27711] (NBP3-13692) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for Apolipoprotein E/ApoE Antibody (GT27711)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Apolipoprotein E/ApoE
Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.
The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.
Alternate Names
APOE
Gene Symbol
APOE
Additional Apolipoprotein E/ApoE Products
Product Documents for Apolipoprotein E/ApoE Antibody (GT27711)
Product Specific Notices for Apolipoprotein E/ApoE Antibody (GT27711)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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