Apolipoprotein E R2/ApoE R2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2216

Novus Biologicals, part of Bio-Techne
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NB100-2216
NB100-2216SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Mouse

Predicted:

Bovine (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Apolipoprotein E R2/ApoE R2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human ApoER2 protein sequence (between residues 863-963). [UniProt# Q14114]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: bovine (95%).

Localization

Type I membrane. Isoforms that contain the exon encoding for a furin-cleavage site are proteolytically processed, leading to a secreted receptor fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein E R2/ApoE R2 Antibody

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Analysis of ApoER2 on mouse brain membrane.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216]

Immunocytochemistry/Immunofluorescence: Apolipoprotein E R2/ApoE R2 Antibody [NB100-2216] - Antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for Apolipoprotein E R2/ApoE R2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:500

SDS-Page

reported in scientific literature (PMID 27810638)

Western Blot

2 ug/ml
Application Notes
A band is seen at ~106 kDa representing the membrane form of ApoER2 in Western Blot. A larger ~130 kDa band may also be seen, representing the glycosylated form of ApoER2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E R2/ApoE R2

ApoER2 (apolipoprotein E receptor 2 or LDL receptor related protein 8) is a member of LDL receptor (LDLR) family and it acts as a cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. ApoER2 facilitates extracellular RELN signal transduction to intracellular signaling processes, mainly through binding with DAB1 on its cytoplasmic tail. RELN acts via both VLDLR and ApoER2 to regulate tyrosine phosphorylation mediated DAB1 activation and microtubule function in neuronal cells. ApoER2 possess higher affinity for RELN compared to VLDLR and is therefore, a key player in Reelin pathway that governs forebrain's neuronal layering during prenatal brain development. ApoER2 associates with ER resident receptor-associated protein (RAP) as well as with dimers of beta 2-glycoprotein I. In the vasculature, ApoER2 has been suggested to implicate in suppressing platelet aggregation. Moreover, ApoER2 is highly expressed in epididymis (initial segment), where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), which are required for sperm maturation. ApoER2 also function as an endocytic receptor and defective ApoER2 have been linked to myocardial infarction type 1 (MCI1).

Long Name

Apolipoprotein E Receptor 2

Alternate Names

ApoER2, Lr8b, LRP-8, LRP8

Gene Symbol

LRP8

Additional Apolipoprotein E R2/ApoE R2 Products

Product Documents for Apolipoprotein E R2/ApoE R2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Apolipoprotein E R2/ApoE R2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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